Protein oxidative damage: a target for efficient dermo-cosmetic treatments

In addition to chronological ageing, the skin, hair and nails are also damaged by environmental stressors (Indoor and Outdoor pollution, Chemical and Irradiation) accelerating this process. Both chronological and environmental-induced ageing are caused by oxidative damage to our macromolecules, in particular to proteins, the main component of our skin, hair shaft and nail.

Claims substantiation

By using targeted and untargeted proteomics approaches, our scientific team provides solutions for efficacy validation, from screening up to clinical studies

Active ingredient screening

Screening of active ingredients or concentrations based on their biological effect on living cells, through semi-quantification of carbonylated proteins (Oxi-Proteome) by fluorimetry, allowing to select the best promising for in depth efficacy studies.

In situ vizualisation of molecular damage protection

Specific labelling and fluorescence imaging of carbonylated proteins (Oxi-Proteome) to evidence the protective effect of active ingredients or finished products on cells, on distinct anatomic compartments (Stratum corneum, epidermis and dermis), or upon Stratum corneum tape stripping.

Quantification of molecular damage protection

Efficacy score of protection of active ingredients or finished products based on the absolute quantification of carbonylated proteins (Oxi-Proteome) (normalization by total proteins), adapted for both : ex vivo and in vivo studies.

Evidencing protective mechanisms of action

Identification of skin proteins specifically protected through the quantification of protein oxidative rate variation upon stress or physiological condition to evidence the signature of protection of active ingredients or finished products.

In situ visualization of key biomarkers

High-resolution fluorescence imaging to further evidence the effect of  active ingredients or finished products on key protein biomarkers involved in skin structure and function, on distinct anatomic compartments.

Evidencing mechanism of action and new biomarkers

Proved tool based on untargeted proteomics approaches for deep analyses to accelerate time to market of novel active ingredients by underlining their mechanisms of action under stress or physiological conditions and by discovering previously unknown biological targets (proteins) involved in skin function.

We offer added value to Expression proteomics studies by interpreting OMICS data focused on skin biology, in a precise and reliable way, ready to use:

Claims substantiation

Product application

By using targeted proteomics approaches we provide innovative efficacy tests based on the evaluation of hair structure (protein) protection.

In situ visualization of molecular damage protection

Specific labelling and fluorescence imaging of oxidized / protected hair keratins to evidence the protective effect of active ingredients or finished products in the whole hair shaft or cuticule and cortex.

Quantification of molecular damage protection

Efficacy score of hair protection from stress by active ingredients or finished products based on the absolute quantification of carbonylated proteins (Oxi-Proteome) (normalization by total proteins).

Claims substantiation

Let’s talk

Our scientific team possesses expertise using in vitro, ex vivo or in vivo models of human skin coupled with innovative analyses at the molecular level.  Working with our customers, we are able to develop and validate also customized projects.  

Because living models are used, we provide a functional read-out of compound activity, or produce results that are more biologically/physiologically relevant than in-tubo assays.

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