Oxidatively-damaged proteins (Oxi-Proteome): a key target to battle for efficient cosmetics

Exposure to environmental stressors inflicts oxidative damage and premature skin aging.  The impact of stress on proteome is of special interest since proteins are key players to assure structure, function and homeostasis in skin, hair and nails. One of the most detrimental consequences of oxidative stress on protein is carbonylation. Interestingly, different patterns of carbonylated proteins in skin, point to the existence of distinct responses, depending on stressors (Read our Technical article « The skin Oxi-Proteome as a molecular signature of exposome stress »), hence the importance of the development of targeted cosmetic products.

By using targeted and untargeted proteomics approaches, our scientific team provides solutions for efficacy validation, from screening up to clinical studies

Active ingredient screening (Oxi-Proteome screen)

Screening of active ingredients or doses based on their biological effect on living cells (keratinocytes, fibroblasts, melanocytes, sensitive neurons) in 96-well plates, through semi-quantification of total carbonylated proteins (Oxi-Proteome) by fluorimetry, allowing to select the best promising to in deep efficacy studies.

In situ visualization of molecular damage protection (Oxi-Proteome view)

Specific labelling and fluorescence imaging of carbonylated proteins (Oxi-Proteome) to evidence the protective effect of active ingredients on cells.

Quantification of molecular damage protection (Oxi-Proteome score)

Efficacy score of protection of active ingredients based on the absolute quantification of carbonylated proteins (Oxi-Proteome) (normalization by total proteins) in cells.

In situ visualization of molecular damage protection (Oxi-Proteome view)

Specific labelling and fluorescence imaging of carbonylated proteins (Oxi-Proteome) to evidence the protective effect of active ingredients or finished products on distinct anatomic compartments (dermis, epidermis, Stratum corneum).

High-resolution imaging is backed by semi-quantification by image analysis in the whole explants and by skin layers.

Quantification of molecular damage protection (Oxi-Proteome score)

Efficacy score of protection of active ingredients or finished products based on the absolute quantification of carbonylated proteins (Oxi-Proteome) (normalization by total proteins) in skin explants.

Evidencing protective mechanisms of action (Identification of protected proteins)

Identification of proteins specifically protected through the quantification of protein oxidative rate variation upon stress or physiological condition to evidence the signature of protection of active ingredients or finished products.

In situ visualization of key biomarkers (Skin structure and function)

High-resolution fluorescence imaging to further evidence the effect of active ingredients or finished products on key protein biomarkers involved in skin structure and function on distinct anatomic compartments.

In situ visualization of molecular damage protection (Oxi-Proteome view)

Specific labelling and fluorescence imaging of carbonylated proteins (Oxi-Proteome) to evidence the protective effect of active ingredients or finished products upon Stratum corneum tape stripping.

Quantification of molecular damage protection (Oxi-Proteome score)

Efficacy score of protection of active ingredients or finished products based on the absolute quantification of carbonylated proteins (Oxi-Proteome) (normalization by total proteins) on skin surface (tape stripping).

Evidencing mechanism of action and new biomarkers

Proved tool based on untargeted proteomics approaches for deep analyses to accelerate time to market of novel active ingredients by underlining their mechanisms of action under stress or physiological conditions and by discovering previously unknown biological targets (proteins) involved in skin function.

We offer added value to Expression proteomics studies by interpreting OMICS data focused on skin biology, in a precise and reliable way, ready to use:

By using targeted proteomics approaches we provide innovative efficacy tests based on the evaluation of hair structure (protein) protection.

In situ visualization of molecular damage protection (Oxi-Proteome view)

Specific labelling and fluorescence imaging of oxidized / protected hair keratins to evidence the protective effect of active ingredients or finished products in the whole hair shaft or cuticule and cortex.

High-resolution imaging is backed by semi-quantification by image analysis.

Quantification of molecular damage protection (Oxi-Proteome score)

Efficacy score of protection of hair from stress by active ingredients or finished products based on the absolute quantification of carbonylated proteins (normalization by total proteins) in the whole hair shaft.

In situ visualization of molecular damage protection (Oxi-Proteome view)

Specific labelling and fluorescence imaging of oxidized / protected hair keratins to evidence the protective effect of active ingredients or finished products in the whole hair shaft or cuticule and cortex.

High-resolution imaging is backed by semi-quantification by image analysis.

Quantification of molecular damage protection (Oxi-Proteome score)

Efficacy score of protection of hair from stress by active ingredients or finished products based on the absolute quantification of carbonylated proteins (normalization by total proteins) in the whole hair shaft.

Let’s talk

Our scientific team possesses expertise using in vitro, ex vivo or in vivo models of human skin coupled with innovative analyses at the molecular level.  Working with our customers, we are able to develop and validate also customized projects.  

Because living models are used, we provide a functional read-out of compound activity, or produce results that are more biologically/physiologically relevant than in-tubo assays.

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