Biomarkers are pivotal in the One Health concept, which emphasizes the interconnectedness of human, animal, and environmental health. By serving as indicators of shared health threats across species and ecosystems, biomarkers play a crucial role in early detection, and intervention efficacy assessment. They enable us to track environmental exposures, and monitor the impact of interventions on both human and animal populations. Biomarker data facilitate collaborative efforts between human and veterinary medicine, environmental science, and public health, ultimately promoting holistic approaches to health management that prioritize the well-being and healthy longevity of humans, animals, and the environment alike.